Whole cell biocatalysis is a methodological approach employing intact or minimally disrupted living cells as catalysts for chemical transformations. This field investigates the application of cellular metabolic networks, including enzymes, cofactors, and transport systems, *in situ* to facilitate the synthesis or modification of organic molecules, often enabling complex, multi-step reactions without enzyme isolation. Its significance lies in leveraging the inherent cellular machinery for efficiency, cofactor regeneration, and compartmentalization, offering a potentially more sustainable and economical alternative to traditional chemical synthesis or isolated enzyme catalysis.